Citiwell OR7500 Service Manual download pdf (Page 3)

Agilent DNA 7500 and DNA 12000 3

Contents

1 Agilent DNA 7500 and DNA 12000 Kit 4

2 Equipment Required for a DNA 7500 and DNA 12000 Assay 5

3 Setting up the Assay Equipment and Bioanalyzer 6

Setting up the Chip Priming Station 7

Setting up the Bioanalyzer 8

Vortex Mixer 8

Starting the 2100 Expert Software 9

4 Essential Measurement Practices 10

5 Agilent DNA 7500 and DNA 12000 Assay Protocol 11

Preparing the Gel-Dye Mix 11

Loading the Gel-Dye Mix 13

Loading the Marker 14

Loading the Ladder and the Samples 15

Inserting a Chip in the Agilent 2100 Bioanalyzer 16

Starting the Chip Run 17

Cleaning Electrodes after a DNA Chip Run 19

6 Checking Your Agilent DNA 7500 and DNA 12000 Assay Results 20

DNA 7500 and DNA 12000 Ladder Well Results 20

DNA 7500 and DNA 12000 Sample Well Results 22

Index 23

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Citiwell OR7500 Service Manual Page 3