Citiwell OR7500 Service Manual download pdf (Page 15)

Agilent DNA 7500 and DNA 12000 15

Agilent DNA 7500 and DNA 12000 Assay Protocol 5

Loading the Ladder and the Samples

1 Pipette 1 µl of the yellow- capped DNA ladder vial

(yellow ) in the well marked with the ladder

symbol .

2 In each of the 12 sample wells pipette 1 µl of

sample (used wells) or 1 µl of deionized water

(unused wells).

3 Set the timer to 60 seconds.

Place the chip horizontally in the adapter of the IKA vortex mixer and

make sure not to damage the buldge that fixes the chip during vortexing.

5 Vortex for 60 seconds at 2400 rpm.

6 Refer to the next topic on how to insert the chip in the Agilent 2100

bioanalyzer. Make sure that the run is started within 5 minutes.

1 µl ladder

1 µl sample

CAUTION

Wrong vortexing speed

If the vortexing speed is too high, liquid spill that disturbs the analysis may occur for

samples generated with detergent containing PCR buffers.

 Reduce vortexing speed to 2000 rpm!

NOTE

For optimal results, samples should be of pH 6 to 9 and should not have an ionic content

greater than twice that of a typical PCR buffer.

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Citiwell OR7500 Service Manual Page 15