Citiwell OR7500 Service Manual download pdf (Page 13)

Agilent DNA 7500 and DNA 12000 13

Agilent DNA 7500 and DNA 12000 Assay Protocol 5

Loading the Gel-Dye Mix

1 Allow the gel- dye mix to equilibrate to room

temperature for 30 minutes before use. Protect

the gel- dye mix from light during this time.

2 Take a new DNA chip out of its sealed bag and

place the chip on the chip priming station.

3 Pipette 9.0 µl of the gel- dye mix at the bottom of

the well marked and dispense the gel- dye

mix.

4 Set the timer to 30 seconds, make sure that the plunger is positioned at

1 ml and then close the chip priming station. The lock of the latch will

click when the chip priming station is closed correctly.

NOTE

Before loading the gel-dye mix, make sure that the base plate of the chip priming station is

in position (C) and the adjustable clip is set to the highest position. Refer to “Setting up the

Chip Priming Station” on page 7 for details.

9 µl gel-dye

NOTE

When pipetting the gel-dye mix, make sure not to draw up particles that may sit at the

bottom of the gel-dye mix vial. Insert the tip of the pipette to the bottom of the chip well

when dispensing. This prevents a large air bubble forming under the gel-dye mix. Placing

the pipette at the edge of the well may lead to poor results.

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Citiwell OR7500 Service Manual Page 13