Citiwell OR7500 Service Manual

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Summary of Contents

Page 1

Agilent TechnologiesAgilent DNA 7500 and DNA 12000 Kit Guide

Page 2

10 10Agilent DNA 7500 and Agilent TechnologiesEssential Measurement Practices• Handle and store all reagents according to the instructions on the la

Page 3 - Contents

11Agilent DNA 7500 and Agilent TechnologiesAgilent DNA 7500 and DNA 12000 Assay ProtocolAfter completing the initial steps in “Setting up the Assay

Page 4

12 Agilent DNA 7500 and DNA 12000 5 Agilent DNA 7500 and DNA 12000 Assay ProtocolPreparing the Gel-Dye Mix2 Vortex the blue- capped vial with DNA dye

Page 5 - DNA 12000 Assay

Agilent DNA 7500 and DNA 12000 13Agilent DNA 7500 and DNA 12000 Assay Protocol 5Loading the Gel-Dye MixLoading the Gel-Dye Mix 1 Allow the gel- dye m

Page 6 - Bioanalyzer

14 Agilent DNA 7500 and DNA 12000 5 Agilent DNA 7500 and DNA 12000 Assay ProtocolLoading the Marker5 Press the plunger of the syringe down until it is

Page 7

Agilent DNA 7500 and DNA 12000 15Agilent DNA 7500 and DNA 12000 Assay Protocol 5Loading the Ladder and the SamplesLoading the Ladder and the Samples

Page 8 - Vortex Mixer

16 Agilent DNA 7500 and DNA 12000 5 Agilent DNA 7500 and DNA 12000 Assay ProtocolInserting a Chip in the Agilent 2100 BioanalyzerInserting a Chip in t

Page 9

Agilent DNA 7500 and DNA 12000 17Agilent DNA 7500 and DNA 12000 Assay Protocol 5Starting the Chip RunStarting the Chip Run 1 In the Instrument contex

Page 10 - Agilent Technologies

18 Agilent DNA 7500 and DNA 12000 5 Agilent DNA 7500 and DNA 12000 Assay ProtocolStarting the Chip Run3Click the Start button in the upper right of th

Page 11 - DNA 12000 Assay Protocol

Agilent DNA 7500 and DNA 12000 19Agilent DNA 7500 and DNA 12000 Assay Protocol 5Cleaning Electrodes after a DNA Chip RunCleaning Electrodes after a D

Page 12 - 25 µl dye

Agilent DNA 7500 and DNA 12000 Notices© Agilent Technologies, Inc. 2000, 2000-2006, 2013No part of this manual may be reproduced in any form or by any

Page 13 - Loading the Gel-Dye Mix

20 20Agilent DNA 7500 and Agilent TechnologiesChecking Your Agilent DNA 7500 and DNA 12000 Assay ResultsDNA 7500 and DNA 12000 Ladder Well ResultsTo

Page 14 - Loading the Marker

Agilent DNA 7500 and DNA 12000 21Checking Your Agilent DNA 7500 and DNA 12000 Assay Results 6DNA 7500 and DNA 12000 Ladder Well ResultsFigure 2 DNA 1

Page 15 - 1 µl sample

22 Agilent DNA 7500 and DNA 12000 6 Checking Your Agilent DNA 7500 and DNA 12000 Assay ResultsDNA 7500 and DNA 12000 Sample Well ResultsDNA 7500 and D

Page 16

Agilent DNA 7500 and DNA 12000 23IndexIndexNumerics2100 expert software, 9, 16Aassay menu, 17Bbase-plate, 7Cchip priming station, 6, 7chip selector,

Page 17 - Starting the Chip Run

© Agilent Technologies, Deutschland GmbH 2000, 2000-2006, 2013Printed in Germany 07/2013*G2938-90024**G2938-90024*G2938-90024 Rev. Bwww.agilent.comAgi

Page 18 - Contamination of electrodes

Agilent DNA 7500 and DNA 12000 3Contents1 Agilent DNA 7500 and DNA 12000 Kit 42 Equipment Required for a DNA 7500 and DNA 12000 Assay 53 Setting up t

Page 19

4 4Agilent DNA 7500 and Agilent TechnologiesAgilent DNA 7500 and DNA 12000 KitAgilent DNA 7500 Kit (reorder number 5067-1506) Agilent DNA 12000 Kit

Page 20 - DNA 12000 Assay Results

5Agilent DNA 7500 and Agilent TechnologiesEquipment Required for a DNA 7500 and DNA 12000 AssayEquipment Supplied with the Agilent 2100 Bioanalyzer•

Page 21 - Lower Marker

6 6Agilent DNA 7500 and Agilent TechnologiesSetting up the Assay Equipment and BioanalyzerBefore beginning the chip preparation protocol, ensure tha

Page 22

Agilent DNA 7500 and DNA 12000 7Setting up the Assay Equipment and Bioanalyzer 3Setting up the Chip Priming StationSetting up the Chip Priming Statio

Page 23

8 Agilent DNA 7500 and DNA 12000 3 Setting up the Assay Equipment and BioanalyzerSetting up the BioanalyzerSetting up the Bioanalyzer Adjust the chip

Page 24 - *G2938-90024*

Agilent DNA 7500 and DNA 12000 9Setting up the Assay Equipment and Bioanalyzer 3Starting the 2100 Expert SoftwareStarting the 2100 Expert SoftwareTo

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